{"wikiid":422884,"langid":"en","namespace":"CATEGORY","title":"Spectrophotometer","revisionid":1172029,"contents_raw":"== Background and Identification ==\n\nA spectrophotometer is a device that measures light\u2019s intensity (or number of photons) absorbed after it passes through a solution. Using a spectrophotometer, you can determine the amount of a known chemical substance by measuring the intensity of the light detected.\n\nThere are two types of spectrophotometer and this depends on the range of wavelength of the light source.\n\nUV-visible spectrophotometer: uses light over the ultraviolet range (185 - 400 nm) and visible range (400 - 700 nm) of electromagnetic radiation\n\nIR spectrophotometer: uses light over the infrared range (700 - 15000 nm) of electromagnetic radiation spectrum (Chemistry Libretexts)\n\nSpectrophotometers are used in a wide variety of scientific fields including Physics, Molecular Biology, Chemistry, Biochemistry, Chemical Engineering and Materials Science.\n\n== Additional Information ==\n\n* Wikipedia: [https:\/\/en.wikipedia.org\/wiki\/Spectrophotometry|Spectrophotometry]\n* Chemistry Libretexts: [https:\/\/chem.libretexts.org\/Bookshelves\/Physical_and_Theoretical_Chemistry_Textbook_Maps\/Supplemental_Modules_(Physical_and_Theoretical_Chemistry)\/Kinetics\/Reaction_Rates\/Experimental_Determination_of_Kinetcs\/Spectrophotometry|Spectrophotometry]","contents_json":{"type":"doc","content":[{"type":"heading","attrs":{"level":2},"content":[{"type":"text","text":"Background and Identification"}]},{"type":"paragraph","content":[{"type":"text","text":"A spectrophotometer is a device that measures light\u2019s intensity (or number of photons) absorbed after it passes through a solution. Using a spectrophotometer, you can determine the amount of a known chemical substance by measuring the intensity of the light detected."}]},{"type":"paragraph","content":[{"type":"text","text":"There are two types of spectrophotometer and this depends on the range of wavelength of the light source."}]},{"type":"paragraph","content":[{"type":"text","text":"UV-visible spectrophotometer: uses light over the ultraviolet range (185 - 400 nm) and visible range (400 - 700 nm) of electromagnetic radiation"}]},{"type":"paragraph","content":[{"type":"text","text":"IR spectrophotometer: uses light over the infrared range (700 - 15000 nm) of electromagnetic radiation spectrum (Chemistry Libretexts)"}]},{"type":"paragraph","content":[{"type":"text","text":"Spectrophotometers are used in a wide variety of scientific fields including Physics, Molecular Biology, Chemistry, Biochemistry, Chemical Engineering and Materials Science."}]},{"type":"heading","attrs":{"level":2},"content":[{"type":"text","text":"Additional Information"}]},{"type":"bulletList","attrs":[],"content":[{"type":"listItem","content":[{"type":"paragraph","content":[{"type":"text","text":"Wikipedia: "},{"type":"text","marks":[{"type":"link","attrs":{"href":"https:\/\/en.wikipedia.org\/wiki\/Spectrophotometry","target":false}}],"text":"Spectrophotometry"}]}]},{"type":"listItem","content":[{"type":"paragraph","content":[{"type":"text","text":"Chemistry Libretexts: "},{"type":"text","marks":[{"type":"link","attrs":{"href":"https:\/\/chem.libretexts.org\/Bookshelves\/Physical_and_Theoretical_Chemistry_Textbook_Maps\/Supplemental_Modules_(Physical_and_Theoretical_Chemistry)\/Kinetics\/Reaction_Rates\/Experimental_Determination_of_Kinetcs\/Spectrophotometry","target":false}}],"text":"Spectrophotometry"}]}]}]}]},"contents_rendered":"
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Background and Identification<\/h2><\/div>\n\n

A spectrophotometer is a device that measures light\u2019s intensity (or number of photons) absorbed after it passes through a solution. Using a spectrophotometer, you can determine the amount of a known chemical substance by measuring the intensity of the light detected.<\/p>\n\n

There are two types of spectrophotometer and this depends on the range of wavelength of the light source.<\/p>\n\n

UV-visible spectrophotometer: uses light over the ultraviolet range (185 - 400 nm) and visible range (400 - 700 nm) of electromagnetic radiation<\/p>\n\n

IR spectrophotometer: uses light over the infrared range (700 - 15000 nm) of electromagnetic radiation spectrum (Chemistry Libretexts)<\/p>\n\n

Spectrophotometers are used in a wide variety of scientific fields including Physics, Molecular Biology, Chemistry, Biochemistry, Chemical Engineering and Materials Science.<\/p>\n\n

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Additional Information<\/h2><\/div>\n\n